Hotspot

rpl36-rps8

LSC · 80469-80982 · 514 bp

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Pi 0.0368

MarkerSeek score 75.00

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 18
pi: 0.0368
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CGGATTAGGCGGCATTTTTC	GAAGGAATCGGCGGAGAAAT	50.0	50.0	57.9	58.0	600-606 bp	1.0	84.4
rpl36-rps8_p2	CTACGGATTAGGCGGCATTT	GAAGGAATCGGCGGAGAAAT	50.0	50.0	58.1	58.0	603-609 bp	1.0	84.3
rpl36-rps8_p3	TACGGATTAGGCGGCATTTT	GAAGGAATCGGCGGAGAAAT	45.0	50.0	57.7	58.0	602-608 bp	1.0	82.6
rpl36-rps8_p4	TTACTATAATTCGCCCCCGC	GAAGGAATCGGCGGAGAAAT	50.0	50.0	57.5	58.0	623-629 bp	1.0	81.4
rpl36-rps8_p5	CGGATTAGGCGGCATTTTTC	AGGAATCGGCGGAGAAATTT	50.0	45.0	57.9	57.6	598-604 bp	1.0	81.4