Hotspot

rpoA-rps11

LSC · 79740-79819 · 80 bp

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Pi 0.0375

MarkerSeek score 71.10

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 80
variable_sites: 3
pi: 0.0375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	GCGGAGACAGAATAAACCGT	TCGTACGAGATGTAACCCCT	50.0	50.0	58.0	57.6	254 bp	1.0	77.5
rpoA-rps11_p2	GCGGAGACAGAATAAACCGT	CGAGATGTAACCCCTATGCC	50.0	55.0	58.0	57.5	249 bp	1.0	77.3
rpoA-rps11_p3	TTCAACACACTTCCACTGCA	TCGTACGAGATGTAACCCCT	45.0	50.0	57.9	57.6	202 bp	1.0	76.8
rpoA-rps11_p4	TTCAACACACTTCCACTGCA	CGAGATGTAACCCCTATGCC	45.0	55.0	57.9	57.5	197 bp	1.0	76.6
rpoA-rps11_p5	ACTGCAGTGTCCGAGTAGAT	TCGTACGAGATGTAACCCCT	50.0	50.0	58.2	57.6	188 bp	1.0	76.6