Hotspot

ndhC-trnV-UAC

LSC · 51622-52058 · 437 bp

Back to Psychotria

Pi 0.0359

MarkerSeek score 69.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 14
pi: 0.0359
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCCCAAAAATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.5	58.7	453-493 bp	1.0	46.4
ndhC-trnV-UAC_p2	ACCCCAAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.5	57.8	465-505 bp	1.0	46.3
ndhC-trnV-UAC_p3	CCCCAAAAATATCATATTCGTAAAGC	AGAAGGTCTACGGTTCGAGT	34.6	50.0	57.3	57.8	464-504 bp	1.0	46.3
ndhC-trnV-UAC_p4	ACCCCAAAAATATCATATTCGT	GAAGGTCTACGGTTCGAGTC	31.8	55.0	53.5	57.5	464-504 bp	1.0	46.3
ndhC-trnV-UAC_p5	CCCCAAAAATATCATATTCGTAAAGC	GAAGGTCTACGGTTCGAGTC	34.6	55.0	57.3	57.5	463-503 bp	1.0	46.3