Hotspot

trnT-UGU-trnL-UAA

LSC · 46134-46851 · 718 bp

Back to Psychotria

Pi 0.0358
MarkerSeek score 79.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
718
variable_sites
24
pi
0.0358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 728-768 bp 1.0 85.5
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG TTTCACTTGGCGAGTTTCCA 55.0 45.0 57.9 58.0 783-823 bp 1.0 85.0
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 736-776 bp 1.0 84.9
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG GTTTCCATACCAAGGCCCAA 55.0 50.0 57.9 58.1 770-810 bp 1.0 84.8
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG TGGCGAGTTTCCATACCAAG 55.0 50.0 57.9 57.9 776-816 bp 1.0 84.5