Hotspot

ycf3-trnS-GGA

LSC · 44362-44687 · 326 bp

Back to Psychotria

Pi 0.046

MarkerSeek score 71.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 326
variable_sites: 15
pi: 0.046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	510-543 bp	1.0	68.4
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	511-544 bp	1.0	68.4
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	460-493 bp	1.0	68.3
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	461-494 bp	1.0	68.3
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	CCCCGGTAAACAAAAGCCTA	45.0	50.0	56.4	57.8	488-521 bp	1.0	68.1