Hotspot

psbM-trnD-GUC

LSC · 29117-29871 · 755 bp

Back to Psychotria

Pi 0.0443

MarkerSeek score 76.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 32
pi: 0.0443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAATAGCGCAGTAGCA	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.5	58.5	866-869 bp	1.0	70.4
psbM-trnD-GUC_p2	GAATGAATAGCGCAGTAGCA	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.0	58.5	865-868 bp	1.0	66.3
psbM-trnD-GUC_p3	AGAATGAATAGCGCAGTAGCA	GTTCAATTGGTCAGAGCACC	42.9	50.0	57.5	57.0	867-870 bp	1.0	66.2
psbM-trnD-GUC_p4	AGAATGAATAGCGCAGTAGCAA	TTCAATTGGTCAGAGCACCG	40.9	50.0	58.2	58.5	866-869 bp	1.0	64.5
psbM-trnD-GUC_p5	AGAATGAATAGCGCAGTAGCA	TCAATTGGTCAGAGCACCG	42.9	52.6	57.5	57.8	865-868 bp	1.0	64.4