Hotspot

trnR-UCU-atpA

LSC · 10165-10285 · 121 bp

Back to Psychotria

Pi 0.0413

MarkerSeek score 69.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 5
pi: 0.0413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAAGCGCCCATCGTCTAATG	AGGAGCAAATGGACCGTTTT	50.0	45.0	58.7	58.0	237 bp	1.0	76.0
trnR-UCU-atpA_p2	CAAAGCGCCCATCGTCTAAT	AGGAGCAAATGGACCGTTTT	50.0	45.0	58.7	58.0	238 bp	1.0	76.0
trnR-UCU-atpA_p3	AAAGCGCCCATCGTCTAATG	ATTCAGGAGCAAATGGACCG	50.0	50.0	58.7	58.2	241 bp	1.0	74.1
trnR-UCU-atpA_p4	CAAAGCGCCCATCGTCTAAT	ATTCAGGAGCAAATGGACCG	50.0	50.0	58.7	58.2	242 bp	1.0	74.1
trnR-UCU-atpA_p5	AAAGCGCCCATCGTCTAATG	AGACATTTACCGAGGAAGCG	50.0	50.0	58.7	57.7	282 bp	1.0	73.5