Hotspot

trnA-UGC-trnI-GAU

IRa · 105036-105301 · 266 bp

Back to Psittacanthus

Pi 0.337

MarkerSeek score 68.50

Primer available yes

Label trnA-UGC-trnI-GAU

Feature Metadata

label_name: trnA-UGC-trnI-GAU
feature_type: igs
region: IRa
length_bp: 266
variable_sites: 134
pi: 0.337
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-trnI-GAU_p1	ATTCGGGAATCTCCGGATCT	CCCGTCCTCAGTTCAGATTG	50.0	55.0	58.0	58.0	1099-1722 bp	1.0	91.7
trnA-UGC-trnI-GAU_p2	ATTCGGGAATCTCCGGATCT	CTAGTAATCGCCGATCAGCC	50.0	55.0	58.0	58.0	1045-1668 bp	1.0	91.7
trnA-UGC-trnI-GAU_p3	ATGTTTCAGTTCGCCAGGTT	CCCGTCCTCAGTTCAGATTG	45.0	55.0	58.0	58.0	1166-1789 bp	1.0	91.7
trnA-UGC-trnI-GAU_p4	ATGTTTCAGTTCGCCAGGTT	CTAGTAATCGCCGATCAGCC	45.0	55.0	58.0	58.0	1112-1735 bp	1.0	91.7
trnA-UGC-trnI-GAU_p5	ATTCGGGAATCTCCGGATCT	AACTCCAAAAACCCGTCCTC	50.0	50.0	58.0	58.0	1110-1733 bp	1.0	91.7