Hotspot

trnN-GUU-trnR-ACG

IRa · 100682-101157 · 476 bp

Back to Psittacanthus

Pi 0.101
MarkerSeek score 81.70
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
476
variable_sites
96
pi
0.101
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 GCTGTTAACTGACTGGTCGT ATCCCTTCTCCCCCATTTCA 50.0 50.0 57.8 58.0 738-837 bp 1.0 90.8
trnN-GUU-trnR-ACG_p2 CGCTGATACCTTCTTCTCGG ATCCCTTCTCCCCCATTTCA 55.0 50.0 57.9 58.0 1235-1360 bp 0.8 82.9
trnN-GUU-trnR-ACG_p3 CGCTGATACCTTCTTCTCGG GCGGAAAAATAGCTCGATGC 55.0 50.0 57.9 58.0 1355-1479 bp 0.8 82.6
trnN-GUU-trnR-ACG_p4 CGCTGATACCTTCTTCTCGG CCCTGTTCTTACATGACCCG 55.0 55.0 57.9 58.0 1671-1809 bp 0.6 71.7
trnN-GUU-trnR-ACG_p5 CGCTGATACCTTCTTCTCGG ATCAATTCGATCAGGCACCC 55.0 50.0 57.9 58.0 1651 bp 0.2 46.8