Hotspot

psaC-ycf1

SSC · 94887-95479 · 593 bp

Back to Psittacanthus

Pi 0.0877

MarkerSeek score 84.80

Primer available yes

Label psaC-ycf1

Feature Metadata

label_name: psaC-ycf1
feature_type: igs
region: SSC
length_bp: 593
variable_sites: 104
pi: 0.0877
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ycf1_p1	CACATTGAGTGCACCCGATA	TGAACCGATTCGTCTGTCTG	50.0	50.0	58.0	57.7	1147-1335 bp	1.0	89.0
psaC-ycf1_p2	CATTGAGTGCACCCGATACA	TGAACCGATTCGTCTGTCTG	50.0	50.0	58.0	57.7	1145-1333 bp	1.0	89.0
psaC-ycf1_p3	TTGAGTGCACCCGATACATG	TGAACCGATTCGTCTGTCTG	50.0	50.0	58.0	57.7	1143-1331 bp	1.0	89.0
psaC-ycf1_p4	ACATTGAGTGCACCCGATAC	TGAACCGATTCGTCTGTCTG	50.0	50.0	58.0	57.7	1146-1334 bp	1.0	89.0
psaC-ycf1_p5	TTGAGTGCACCCGATACATG	GAACCGATTCGTCTGTCTGT	50.0	50.0	58.0	57.7	1142-1330 bp	1.0	89.0