Hotspot

trnR-ACG-trnN-GUU

IRb · 92404-92879 · 476 bp

Back to Psittacanthus

Pi 0.0956

MarkerSeek score 81.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 476
variable_sites: 92
pi: 0.0956
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	GCGGAAAAATAGCTCGATGC	GCTGCATAGCCCAATAAGGT	50.0	50.0	58.0	58.0	1516-2039 bp	0.6	71.3
trnR-ACG-trnN-GUU_p2	ATCCCTTCTCCCCCATTTCA	GCTGCATAGCCCAATAAGGT	50.0	50.0	58.0	58.0	1396-1928 bp	0.6	71.1
trnR-ACG-trnN-GUU_p3	ATCCCTTCTCCCCCATTTCA	ATAGCGGCATTGTTCCTCAG	50.0	50.0	58.0	58.0	780-859 bp	0.4	64.6
trnR-ACG-trnN-GUU_p4	CCCTGTTCTTACATGACCCG	GCTGCATAGCCCAATAAGGT	55.0	50.0	58.0	58.0	1832 bp	0.2	47.7
trnR-ACG-trnN-GUU_p5	ATCAATTCGATCAGGCACCC	GCTGCATAGCCCAATAAGGT	50.0	50.0	58.0	58.0	1812 bp	0.2	47.7