Hotspot

trnI-GAU-trnA-UGC

IRb · 88260-88525 · 266 bp

Back to Psittacanthus

Pi 0.325
MarkerSeek score 68.50
Primer available yes
Label trnI-GAU-trnA-UGC

Feature Metadata

label_name
trnI-GAU-trnA-UGC
feature_type
igs
region
IRb
length_bp
266
variable_sites
131
pi
0.325
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.68
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-trnA-UGC_p1 CCCGTCCTCAGTTCAGATTG ATTCGGGAATCTCCGGATCT 55.0 50.0 58.0 58.0 1099-1722 bp 1.0 91.7
trnI-GAU-trnA-UGC_p2 CTAGTAATCGCCGATCAGCC ATTCGGGAATCTCCGGATCT 55.0 50.0 58.0 58.0 1045-1668 bp 1.0 91.7
trnI-GAU-trnA-UGC_p3 CCCGTCCTCAGTTCAGATTG ATGTTTCAGTTCGCCAGGTT 55.0 45.0 58.0 58.0 1166-1789 bp 1.0 91.7
trnI-GAU-trnA-UGC_p4 CTAGTAATCGCCGATCAGCC ATGTTTCAGTTCGCCAGGTT 55.0 45.0 58.0 58.0 1112-1735 bp 1.0 91.7
trnI-GAU-trnA-UGC_p5 AACTCCAAAAACCCGTCCTC ATTCGGGAATCTCCGGATCT 50.0 50.0 58.0 58.0 1110-1733 bp 1.0 91.7