Hotspot

psbE-petL

LSC · 53574-54500 · 927 bp

Back to Psittacanthus

Pi 0.0535

MarkerSeek score 83.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 927
variable_sites: 102
pi: 0.0535
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TCGTTCGGGTTTGATTGTGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1853-1987 bp	1.0	89.7
psbE-petL_p2	ACGGTAGGTACAGCTAGTCC	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.0	58.0	1762-1896 bp	1.0	89.7
psbE-petL_p3	GGTAGGTACAGCTAGTCCGT	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.0	58.0	1760-1894 bp	1.0	89.7
psbE-petL_p4	ATGGAATTCCTTGTCGGCTC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	1620-1704 bp	0.8	81.0
psbE-petL_p5	TTCGGGTTTGATTGTGTCGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1850-1984 bp	0.8	80.4