Hotspot

ycf3-trnS-GGA

LSC · 38654-39308 · 655 bp

Back to Psittacanthus

Pi 0.0488

MarkerSeek score 80.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 63
pi: 0.0488
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CTTCAACAAGCGCGGATTTT	GGAGCCCGCCAATTAGTTAA	45.0	50.0	57.9	58.0	1406-1483 bp	1.0	89.3
ycf3-trnS-GGA_p2	TGAGCGGGGCTAATGTTTTT	GGAGCCCGCCAATTAGTTAA	45.0	50.0	58.1	58.0	1927-1985 bp	1.0	88.5
ycf3-trnS-GGA_p3	CTTCAACAAGCGCGGATTTT	TTTTCATTTCTTGTCCGCGC	45.0	45.0	57.9	58.0	1068-1121 bp	0.8	79.7
ycf3-trnS-GGA_p4	TGAGCGGGGCTAATGTTTTT	TTTTCATTTCTTGTCCGCGC	45.0	45.0	58.1	58.0	1563-1622 bp	0.8	78.9
ycf3-trnS-GGA_p5	ACAGAAGGGGTTGTGCAATT	TTTTCATTTCTTGTCCGCGC	45.0	45.0	57.9	58.0	1145 bp	0.2	46.9