Hotspot

rpoB-trnC-GCA

LSC · 22477-23277 · 801 bp

Back to Psittacanthus

Pi 0.0592

MarkerSeek score 82.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 93
pi: 0.0592
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCGGGGCTTTGCAATATTTG	TATAGTTATAGCCCGGCCCC	50.0	55.0	58.1	58.1	1286-1490 bp	0.8	79.6
rpoB-trnC-GCA_p2	CACGAGCCCATATCCTTTCC	TATAGTTATAGCCCGGCCCC	55.0	55.0	58.1	58.1	1408-1612 bp	0.8	79.0
rpoB-trnC-GCA_p3	AAGACTAGAGCCCCGCTTAT	TATAGTTATAGCCCGGCCCC	50.0	55.0	57.9	58.1	1616-1820 bp	0.8	78.9
rpoB-trnC-GCA_p4	ATGCGAACCCATAGCTGATG	TATAGTTATAGCCCGGCCCC	50.0	55.0	58.1	58.1	1464-1668 bp	0.8	78.9
rpoB-trnC-GCA_p5	AAAGACTAGAGCCCCGCTTA	TATAGTTATAGCCCGGCCCC	50.0	55.0	58.1	58.1	1617-1821 bp	0.8	78.5