Hotspot

rps3

LSC · 86370-87020 · 651 bp

Back to Psidium

Pi 0.00922

MarkerSeek score 64.00

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 651
variable_sites: 6
pi: 0.00922
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	AGTGTAAAGAGTAAAGACAAGGA	AAAGAAGTACACAGATATGACA	34.8	31.8	55.0	52.6	733-734 bp	1.0	40.7
rps3_p2	AGTGTAAAGAGTAAAGACAAGGA	AAAAGAAGTACACAGATATGACA	34.8	30.4	55.0	53.4	734-735 bp	1.0	40.7
rps3_p3	AGTGTAAAGAGTAAAGACAAGGA	AAGAAGTACACAGATATGACAT	34.8	31.8	55.0	52.4	732-733 bp	1.0	40.7
rps3_p4	AGTGTAAAGAGTAAAGACAAGGAA	AAAGAAGTACACAGATATGACA	33.3	31.8	55.7	52.6	733-734 bp	1.0	40.7
rps3_p5	AGTGTAAAGAGTAAAGACAAGGA	AAAAAGAAGTACACAGATATGACA	34.8	29.2	55.0	54.2	735-736 bp	1.0	40.7