Hotspot

rpl32-trnL-UAG

SSC · 117662-118400 · 739 bp

Back to Psidium

Pi 0.0178

MarkerSeek score 61.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 739
variable_sites: 13
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGAAGTCTCTTTCTACCGG	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	814-819 bp	1.0	81.4
rpl32-trnL-UAG_p2	GCGAAGTCTCTTTCTACCGG	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	815-820 bp	1.0	81.4
rpl32-trnL-UAG_p3	TCTATTGGGCAGCGTTGAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	846-851 bp	1.0	80.5
rpl32-trnL-UAG_p4	TCTATTGGGCAGCGTTGAAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	847-852 bp	1.0	80.5
rpl32-trnL-UAG_p5	GCGAAGTCTCTTTCTACCGG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.1	57.9	824-829 bp	1.0	80.4