Hotspot

rpl14-rpl16

LSC · 84689-84819 · 131 bp

Back to Psidium

Pi 0.04

MarkerSeek score 79.70

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 5
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GCTGTTATCCGCTACGTTCA	CAATAGCGGCGTCCAAAATG	50.0	50.0	58.1	58.2	220-226 bp	1.0	81.9
rpl14-rpl16_p2	ACACATTAATTCTCGGGCCC	CAATAGCGGCGTCCAAAATG	50.0	50.0	57.9	58.2	241-247 bp	1.0	81.8
rpl14-rpl16_p3	ATCGGCGATTACTAGCTCCT	CAATAGCGGCGTCCAAAATG	50.0	50.0	58.1	58.2	272-278 bp	1.0	81.1
rpl14-rpl16_p4	ACACATTAATTCTCGGGCCC	ATTTCAATAGCGGCGTCCAA	50.0	45.0	57.9	58.3	245-251 bp	1.0	81.1
rpl14-rpl16_p5	ACACATTAATTCTCGGGCCC	TCAATAGCGGCGTCCAAAAT	50.0	45.0	57.9	58.3	242-248 bp	1.0	81.1