Hotspot

clpP-psbB

LSC · 75803-76207 · 405 bp

Back to Psidium

Pi 0.0222

MarkerSeek score 62.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 405
variable_sites: 9
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	AAACGGCCGGGATCATTTAA	50.0	45.0	58.0	57.9	537-539 bp	1.0	80.3
clpP-psbB_p2	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	501-503 bp	1.0	79.5
clpP-psbB_p3	CGCACTATACGTCAACCCAA	AACGGCCGGGATCATTTAAT	50.0	45.0	58.0	57.6	536-538 bp	1.0	78.5
clpP-psbB_p4	CGCACTATACGTCAACCCAA	AGCAACCAAAGCTGTATGCA	50.0	45.0	58.0	58.4	574-576 bp	1.0	78.2
clpP-psbB_p5	TCCTCTCCAGGACTTCGAAA	AAACGGCCGGGATCATTTAA	50.0	45.0	57.7	57.9	508-510 bp	1.0	77.9