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psbE-petL

LSC · 68330-69574 · 1245 bp

Back to Psidium

Pi 0.0212

MarkerSeek score 72.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1245
variable_sites: 26
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGGTCAGACC	50.0	50.0	57.9	56.4	1438-1440 bp	1.0	69.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1391-1393 bp	1.0	68.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGGTCAGACC	50.0	50.0	57.8	56.4	1361-1363 bp	1.0	68.5
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AGTCGTATCTTGGTCAGACC	45.0	50.0	58.2	56.4	1447-1449 bp	1.0	68.2
psbE-petL_p5	TGCTGACGAATAACCAACCC	AGTCGTATCTTGGTCAGACC	50.0	50.0	58.2	56.4	1452-1454 bp	1.0	68.1