Hotspot

ycf4-cemA

LSC · 63723-64671 · 949 bp

Back to Psidium

Pi 0.00842

MarkerSeek score 66.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 7
pi: 0.00842
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGACTCCGCGTGAAATTGA	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	984-1095 bp	1.0	82.9
ycf4-cemA_p2	AGAATTTGACTCCGCGTGAA	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	989-1100 bp	1.0	82.6
ycf4-cemA_p3	TATTTCTTGCGCGTACCGAT	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	942-1053 bp	1.0	82.2
ycf4-cemA_p4	CCGCGTGAAATTGAGCAAAA	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.2	58.1	978-1089 bp	1.0	82.0
ycf4-cemA_p5	GAGAATTTGACTCCGCGTGA	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.3	58.1	990-1101 bp	1.0	81.6