Hotspot

ndhK-ndhC

LSC · 53611-53664 · 54 bp

Back to Psidium

Pi 0.037

MarkerSeek score 67.80

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 54
variable_sites: 2
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	CGGCCATAAACTAGAGAGCC	CATGGCGAAAAGGAGCATTG	55.0	50.0	57.8	58.1	194-196 bp	1.0	78.9
ndhK-ndhC_p2	TTTGGGTTGTTCGAGCAAGT	CATGGCGAAAAGGAGCATTG	45.0	50.0	58.2	58.1	129-131 bp	1.0	78.6
ndhK-ndhC_p3	CGGCCATAAACTAGAGAGCC	TGGGCAATGAGTTTCGATGT	55.0	45.0	57.8	57.8	282-284 bp	1.0	77.7
ndhK-ndhC_p4	TTTGGGTTGTTCGAGCAAGT	TGGGCAATGAGTTTCGATGT	45.0	45.0	58.2	57.8	217-219 bp	1.0	77.2
ndhK-ndhC_p5	CGGCCATAAACTAGAGAGCC	CGATGTATTGGGGGTATCCG	55.0	55.0	57.8	57.6	268-270 bp	1.0	76.3