Hotspot

trnF-GAA-ndhJ

LSC · 51549-52335 · 787 bp

Back to Psidium

Pi 0.0131

MarkerSeek score 66.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 10
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	912-929 bp	1.0	82.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	876-893 bp	1.0	81.8
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	900-917 bp	1.0	81.0
trnF-GAA-ndhJ_p4	AAGGAATGGTCGGGATAGCT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.2	58.1	923-940 bp	1.0	80.5
trnF-GAA-ndhJ_p5	TGTGTAAGGAATGGTCGGGA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.4	58.1	928-945 bp	1.0	79.2