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psbZ-trnG-GCC

LSC · 38597-39459 · 863 bp

Back to Psidium

Pi 0.0133

MarkerSeek score 62.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 11
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GACGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	986-1009 bp	1.0	81.2
psbZ-trnG-GCC_p2	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	998-1021 bp	1.0	79.0
psbZ-trnG-GCC_p3	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	999-1022 bp	1.0	77.7
psbZ-trnG-GCC_p4	ACGGTTGGTCGAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	985-1008 bp	1.0	77.5
psbZ-trnG-GCC_p5	GACGGTTGGTCGAGTAACAA	ACTTTAACCTGGGCGGATAG	50.0	50.0	57.9	57.0	1021-1044 bp	1.0	74.4