Hotspot

trnT-GGU-psbD

LSC · 33931-35296 · 1366 bp

Back to Psidium

Pi 0.00878

MarkerSeek score 57.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1366
variable_sites: 12
pi: 0.00878
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACGGTCTCTACGTAACCAGT	55.0	50.0	58.1	57.8	1505-1542 bp	1.0	78.6
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.1	57.8	1508-1545 bp	1.0	78.4
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.3	57.8	1482-1519 bp	1.0	76.8
trnT-GGU-psbD_p4	TGGGCCCTTTTAACTCAGTG	ACGGTCTCTACGTAACCAGT	50.0	50.0	57.7	57.8	1522-1559 bp	1.0	76.8
trnT-GGU-psbD_p5	GGCCCTTTTAACTCAGTGGT	ACGGTCTCTACGTAACCAGT	50.0	50.0	57.7	57.8	1520-1557 bp	1.0	76.8