Hotspot

rpoC1-rpoB

LSC · 24799-24803 · 5 bp

Back to Psidium

Pi 0.2

MarkerSeek score 87.50

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 5
variable_sites: 1
pi: 0.2
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TGGCCCAAGCACTTATTTGT	TTGCTCGTTCGAGAACTACG	45.0	50.0	58.0	58.0	192 bp	1.0	80.0
rpoC1-rpoB_p2	TGGCCCAAGCACTTATTTGT	CTTTGGCCCTGGAACTGAAT	45.0	50.0	58.0	58.1	170 bp	1.0	79.8
rpoC1-rpoB_p3	TGGCCCAAGCACTTATTTGT	TTTGGCCCTGGAACTGAATC	45.0	50.0	58.0	58.1	169 bp	1.0	79.8
rpoC1-rpoB_p4	TGGCCCAAGCACTTATTTGT	GGCCCTGGAACTGAATCATT	45.0	50.0	58.0	57.9	166 bp	1.0	79.2
rpoC1-rpoB_p5	TGGCCCAAGCACTTATTTGT	TTCGATTGCTCGTTCGAGAA	45.0	45.0	58.0	57.7	197 bp	1.0	77.6