Hotspot

rps2-rpoC2

LSC · 17474-17700 · 227 bp

Back to Psidium

Pi 0.0441

MarkerSeek score 77.80

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 10
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	ACCAAAATGAACTCCCGCTT	TTCGAGGGGGAAATCAGAGA	45.0	50.0	58.0	57.8	407 bp	1.0	79.9
rps2-rpoC2_p2	AATGAACTCCCGCTTCCATC	TTCGAGGGGGAAATCAGAGA	50.0	50.0	58.0	57.8	402 bp	1.0	79.7
rps2-rpoC2_p3	CCCGCTTCCATCATCTCTTC	TTCGAGGGGGAAATCAGAGA	55.0	50.0	58.1	57.8	394 bp	1.0	79.1
rps2-rpoC2_p4	CCGCTTCCATCATCTCTTCC	TTCGAGGGGGAAATCAGAGA	55.0	50.0	58.1	57.8	393 bp	1.0	79.1
rps2-rpoC2_p5	ACCAAAATGAACTCCCGCTT	TCGAGGGGGAAATCAGAGAT	45.0	50.0	58.0	57.5	406 bp	1.0	78.1