Hotspot

trnS-GCU-trnG-UCC

LSC · 8857-9638 · 782 bp

Back to Psidium

Pi 0.0219

MarkerSeek score 60.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 17
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	903-905 bp	1.0	52.4
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	904-906 bp	1.0	50.4
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	889-891 bp	1.0	48.6
trnS-GCU-trnG-UCC_p4	CTTTCGTCCACTCAGCCATC	ACGAATCACACTTTTACCACT	55.0	38.1	58.6	55.5	839-841 bp	1.0	48.6
trnS-GCU-trnG-UCC_p5	AACGGAAAGAGAGGGATTCG	ACGAATCACACTTTTACCACT	50.0	38.1	57.3	55.5	904-906 bp	1.0	48.2