Hotspot

trnH-GUG-psbA

LSC · 86-557 · 472 bp

Back to Psidium

Pi 0.0237

MarkerSeek score 63.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 472
variable_sites: 11
pi: 0.0237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	532-539 bp	1.0	81.1
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	546-553 bp	1.0	81.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	545-552 bp	1.0	81.0
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	547-554 bp	1.0	81.0
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	535-542 bp	1.0	81.0