Hotspot

petL

LSC · 69610-69705 · 96 bp

Back to Pseudoxandra

Pi 0.0312

MarkerSeek score 69.00

Primer available yes

Label petL

Feature Metadata

label_name: petL
feature_type: gene
region: LSC
length_bp: 96
variable_sites: 3
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL_p1	TCTTATCCTGACTATTTCGCA	ACGCGGGAAGGAACTATAGA	38.1	50.0	54.2	57.9	267 bp	1.0	41.9
petL_p2	TCCTGACTATTTCGCAAAAA	ACGCGGGAAGGAACTATAGA	35.0	50.0	53.1	57.9	262 bp	1.0	41.5
petL_p3	TCTTATCCTGACTATTTCGCA	TGACGCGGGAAGGAACTATA	38.1	50.0	54.2	58.2	269 bp	1.0	40.9
petL_p4	TCTTATCCTGACTATTTCGCAA	ACGCGGGAAGGAACTATAGA	36.4	50.0	55.0	57.9	267 bp	1.0	40.9
petL_p5	TTCTTATCCTGACTATTTCGCA	ACGCGGGAAGGAACTATAGA	36.4	50.0	55.0	57.9	268 bp	1.0	40.9