Hotspot

accD

LSC · 57484-59424 · 1941 bp

Back to Pseudoxandra

Pi 0.0222

MarkerSeek score 63.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1941
variable_sites: 41
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ACGAAATAGATGTATGATAATCCGC	TAATGAGTTAGAACGCGGGC	36.0	50.0	57.2	58.1	2023 bp	1.0	45.2
accD_p2	CGAAATAGATGTATGATAATCCGCT	TAATGAGTTAGAACGCGGGC	36.0	50.0	57.0	58.1	2022 bp	1.0	45.2
accD_p3	CGAAATAGATGTATGATAATCCGC	TAATGAGTTAGAACGCGGGC	37.5	50.0	55.7	58.1	2022 bp	1.0	45.2
accD_p4	CACGAAATAGATGTATGATAATCCGC	TAATGAGTTAGAACGCGGGC	38.5	50.0	58.4	58.1	2024 bp	1.0	45.2
accD_p5	CGAAATAGATGTATGATAATCCGCTT	TAATGAGTTAGAACGCGGGC	34.6	50.0	57.6	58.1	2022 bp	1.0	45.2