Hotspot

ccsA-ndhD

SSC · 120211-120429 · 219 bp

Back to Pseudoxandra

Pi 0.0467
MarkerSeek score 70.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
219
variable_sites
10
pi
0.0467
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTTGGGGTCA TCAGTTGACAAGGCAGAAACT 40.9 42.9 57.2 57.7 345 bp 1.0 50.8
ccsA-ndhD_p2 TGGATATGCTATTTTGGGGTCAA TCAGTTGACAAGGCAGAAACT 39.1 42.9 57.9 57.7 345 bp 1.0 48.1
ccsA-ndhD_p3 TTGGATATGCTATTTTGGGGTCA TCAGTTGACAAGGCAGAAACT 39.1 42.9 57.9 57.7 346 bp 1.0 48.1
ccsA-ndhD_p4 TGGATATGCTATTTTGGGGTCA TCAGTTGACAAGGCAGAAAC 40.9 45.0 57.2 56.2 345 bp 1.0 46.6
ccsA-ndhD_p5 TGGATATGCTATTTTGGGGTCA CAGTTGACAAGGCAGAAACT 40.9 45.0 57.2 56.2 344 bp 1.0 46.5