Hotspot

trnN-GUU-ndhF

IRb · 114142-114880 · 739 bp

Back to Pseudoxandra

Pi 0.0522

MarkerSeek score 72.40

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 739
variable_sites: 31
pi: 0.0522
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AAGCAAAAATGCGTTCCTCG	CGTCGGGTCCATAGGAATTC	45.0	55.0	57.9	57.8	1710 bp	0.5	58.4
trnN-GUU-ndhF_p2	AAGCAAAAATGCGTTCCTCG	TTACTTTGTTCGTCGGGTCC	45.0	50.0	57.9	57.8	1720 bp	0.5	58.4
trnN-GUU-ndhF_p3	GAACTTGGGTGTGGGTCTTT	CGTCGGGTCCATAGGAATTC	50.0	55.0	57.9	57.8	1901 bp	0.5	58.3
trnN-GUU-ndhF_p4	GAACTTGGGTGTGGGTCTTT	TTACTTTGTTCGTCGGGTCC	50.0	50.0	57.9	57.8	1911 bp	0.5	58.2
trnN-GUU-ndhF_p5	AAGCAAAAATGCGTTCCTCG	TCGTCGGGTCCATAGGAATT	45.0	50.0	57.9	58.2	1711 bp	0.5	57.9