Hotspot

ycf2-trnL-CAA

IRb · 98194-99236 · 1043 bp

Back to Pseudoxandra

Pi 0.0182

MarkerSeek score 69.20

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 1043
variable_sites: 19
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	AGGCATTGTGGAGAAAGAGA	AAATGGTAGACACGCGAGAC	45.0	50.0	56.4	58.0	1204 bp	1.0	68.7
ycf2-trnL-CAA_p2	AGGCATTGTGGAGAAAGAGA	TGAAATGGTAGACACGCGAG	45.0	50.0	56.4	58.0	1206 bp	1.0	68.7
ycf2-trnL-CAA_p3	AGGAGAAGGATTCCCCATTC	AAATGGTAGACACGCGAGAC	50.0	50.0	56.3	58.0	1137 bp	1.0	67.4
ycf2-trnL-CAA_p4	AGGAGAAGGATTCCCCATTC	TGAAATGGTAGACACGCGAG	50.0	50.0	56.3	58.0	1139 bp	1.0	67.4
ycf2-trnL-CAA_p5	AGGCATTGTGGAGAAAGAGA	TGGTGAAATGGTAGACACGC	45.0	50.0	56.4	58.2	1209 bp	1.0	67.2