Hotspot

rps19-rpl2

IRb · 88599-88668 · 70 bp

Back to Pseudoxandra

Pi 0.0571

MarkerSeek score 68.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 70
variable_sites: 4
pi: 0.0571
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TTTTTGGGGTAAGCGTGTCA	GTTATCCTGCGCTTGGAAGA	45.0	50.0	57.9	58.0	168-172 bp	1.0	81.7
rps19-rpl2_p2	TTTTTGGGGTAAGCGTGTCA	GGGTAGAGCCGGATCTAAGT	45.0	55.0	57.9	58.0	305-309 bp	1.0	80.9
rps19-rpl2_p3	TTTTTGGGGTAAGCGTGTCA	TGGGTAGAGCCGGATCTAAG	45.0	55.0	57.9	58.0	306-310 bp	1.0	80.8
rps19-rpl2_p4	TTTTTGGGGTAAGCGTGTCA	CGGCCACGTTAAGATTACCA	45.0	50.0	57.9	58.0	408-412 bp	1.0	80.4
rps19-rpl2_p5	TTGAAAGTCAGAGTAGGCGC	GGGTAGAGCCGGATCTAAGT	50.0	55.0	57.9	58.0	526-530 bp	1.0	80.3