Hotspot

pafII-psaI

LSC · 56740-57194 · 455 bp

Back to Pseudoxandra

Pi 0.0155

MarkerSeek score 65.30

Primer available yes

Label pafII-psaI

Feature Metadata

label_name: pafII-psaI
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 7
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-psaI_p1	CAGGCCCAGCAGAAATTACT	ATTTCCGGCAATTGCAATGG	50.0	45.0	57.9	58.0	593-595 bp	1.0	80.1
pafII-psaI_p2	AAAAAGGATACAGGCCCAGC	ATTTCCGGCAATTGCAATGG	50.0	45.0	58.1	58.0	603-605 bp	1.0	79.9
pafII-psaI_p3	ACAGGCCCAGCAGAAATTAC	ATTTCCGGCAATTGCAATGG	50.0	45.0	58.2	58.0	594-596 bp	1.0	79.8
pafII-psaI_p4	CAGGCCCAGCAGAAATTACT	GGCAATTGCAATGGCTTCTT	50.0	45.0	57.9	57.9	587-589 bp	1.0	79.5
pafII-psaI_p5	AAAAAGGATACAGGCCCAGC	GGCAATTGCAATGGCTTCTT	50.0	45.0	58.1	57.9	597-599 bp	1.0	79.3