Hotspot

psbM-trnD-GUC

LSC · 31373-32579 · 1207 bp

Back to Pseudoxandra

Pi 0.0146

MarkerSeek score 64.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1207
variable_sites: 17
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	58.2	57.9	1276-1320 bp	1.0	73.2
psbM-trnD-GUC_p2	GAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	56.7	57.9	1275-1319 bp	1.0	73.1
psbM-trnD-GUC_p3	GAATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	57.5	57.9	1275-1319 bp	1.0	70.8
psbM-trnD-GUC_p4	AGAATGAACAACGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	56.5	57.9	1276-1320 bp	1.0	70.7
psbM-trnD-GUC_p5	AATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	40.0	50.0	56.3	57.9	1274-1318 bp	1.0	69.3