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atpI-rps2

LSC · 16380-16592 · 213 bp

Back to Pseudoxandra

Pi 0.0192

MarkerSeek score 65.80

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 4
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	ATGGACTTGGAAACCCCCTA	TTTCGATTCCAGCCAACGAT	50.0	45.0	57.9	57.9	426-431 bp	1.0	80.3
atpI-rps2_p2	GAAATGCTGGCCTACTTCCA	TTTCGATTCCAGCCAACGAT	50.0	45.0	57.9	57.9	396-401 bp	1.0	79.9
atpI-rps2_p3	AAATGCTGGCCTACTTCCAC	TTTCGATTCCAGCCAACGAT	50.0	45.0	58.2	57.9	395-400 bp	1.0	79.7
atpI-rps2_p4	ATGGACTTGGAAACCCCCTA	AGAGCCTCAGTCCGATTGAT	50.0	50.0	57.9	58.2	400-405 bp	1.0	79.5
atpI-rps2_p5	TTCCACACCGGATAGATCGT	TTTCGATTCCAGCCAACGAT	50.0	45.0	58.2	57.9	381-386 bp	1.0	79.2