Hotspot

rps12

Genome · 111047-111847 · 801 bp

Back to Pseudotsuga

Pi 0.00583

MarkerSeek score 62.10

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: Genome
length_bp: 801
variable_sites: 7
pi: 0.00583
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	ATCGAACGAGAAGCCGTATG	GTACTTCGCCGTGACATGAG	50.0	55.0	57.9	58.7	955 bp	1.0	73.8
rps12_p2	AAGCCGTATGAGGTGCAAAT	GTACTTCGCCGTGACATGAG	45.0	55.0	57.9	58.7	945 bp	1.0	73.7
rps12_p3	GAACGAGAAGCCGTATGAGG	GTACTTCGCCGTGACATGAG	55.0	55.0	58.2	58.7	952 bp	1.0	73.5
rps12_p4	ATCGAACGAGAAGCCGTATG	GCCGTGACATGAGTGTGAAA	50.0	50.0	57.9	58.8	948 bp	1.0	73.4
rps12_p5	AAGCCGTATGAGGTGCAAAT	GCCGTGACATGAGTGTGAAA	45.0	50.0	57.9	58.8	938 bp	1.0	73.4