Hotspot

rps15-psaC

Genome · 103406-104740 · 1335 bp

Back to Pseudotsuga

Pi 0.007

MarkerSeek score 61.60

Primer available yes

Label rps15-psaC

Feature Metadata

label_name: rps15-psaC
feature_type: igs
region: Genome
length_bp: 1335
variable_sites: 14
pi: 0.007
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-psaC_p1	GGGCAAACGTAAGCAACTTC	GTACGCATTGGGTACAACCA	50.0	50.0	58.0	58.2	1493-1503 bp	1.0	78.9
rps15-psaC_p2	TTTGGGCAAACGTAAGCAAC	GTACGCATTGGGTACAACCA	45.0	50.0	57.8	58.2	1496-1506 bp	1.0	77.6
rps15-psaC_p3	GGGCAAACGTAAGCAACTTC	ATCTGTGGGACAAGCTCGTA	50.0	50.0	58.0	58.4	1510-1520 bp	1.0	76.9
rps15-psaC_p4	TTTGGGCAAACGTAAGCAAC	ATCTGTGGGACAAGCTCGTA	45.0	50.0	57.8	58.4	1513-1523 bp	1.0	75.6
rps15-psaC_p5	AAATTGGGTATTCGTGGGCT	GTACGCATTGGGTACAACCA	45.0	50.0	57.5	58.2	1423-1433 bp	1.0	75.1