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chlN-rps15

Genome · 96626-103138 · 6513 bp

Back to Pseudotsuga

Pi 0.00491

MarkerSeek score 41.30

Primer available yes

Label chlN-rps15

Feature Metadata

label_name: chlN-rps15
feature_type: igs
region: Genome
length_bp: 6513
variable_sites: 48
pi: 0.00491
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-rps15_p1	ACAAAAATGATCGCTCTGT	ATAATGGGCCATGGTTGCTC	36.8	50.0	52.8	58.3	214-241 bp	1.0	42.8
chlN-rps15_p2	ACAAAAATGATCGCTCTGT	CATAATGGGCCATGGTTGCT	36.8	50.0	52.8	58.6	215-242 bp	1.0	42.7
chlN-rps15_p3	ACAAAAATGATCGCTCTGT	ACATAATGGGCCATGGTTGC	36.8	50.0	52.8	58.9	216-243 bp	1.0	42.7
chlN-rps15_p4	ACAAAAATGATCGCTCTGT	ATCGTATCGGCCGATAAGCT	36.8	50.0	52.8	58.8	252-279 bp	1.0	42.4
chlN-rps15_p5	ACAAAAATGATCGCTCTGT	TATCGTATCGGCCGATAAGC	36.8	50.0	52.8	57.2	253-280 bp	1.0	42.4