Hotspot

tRNA-Met-tRNA-Gly

Genome · 80052-80216 · 165 bp

Back to Pseudotsuga

Pi 0.0218

MarkerSeek score 67.70

Primer available yes

Label tRNA-Met-tRNA-Gly

Feature Metadata

label_name: tRNA-Met-tRNA-Gly
feature_type: igs
region: Genome
length_bp: 165
variable_sites: 5
pi: 0.0218
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Met-tRNA-Gly_p1	CTTGAGGTCACGGGTTCAAA	ATGCGGGTATGGTTGAATGG	50.0	50.0	58.0	58.3	259-271 bp	1.0	82.4
tRNA-Met-tRNA-Gly_p2	CTTGAGGTCACGGGTTCAAA	GCGGGTATGGTTGAATGGTA	50.0	50.0	58.0	57.7	257-269 bp	1.0	82.2
tRNA-Met-tRNA-Gly_p3	TGCGGAGTAGAGTAGTCTGG	ATGCGGGTATGGTTGAATGG	55.0	50.0	58.0	58.3	299-311 bp	1.0	81.7
tRNA-Met-tRNA-Gly_p4	GCGGAGTAGAGTAGTCTGGT	ATGCGGGTATGGTTGAATGG	55.0	50.0	58.0	58.3	298-310 bp	1.0	81.7
tRNA-Met-tRNA-Gly_p5	GGTGCGGAGTAGAGTAGTCT	ATGCGGGTATGGTTGAATGG	55.0	50.0	58.0	58.3	301-313 bp	1.0	81.7