Hotspot

tRNA-Ile-tRNA-Phe

Genome · 66970-68910 · 1941 bp

Back to Pseudotsuga

Pi 0.00996

MarkerSeek score 57.10

Primer available yes

Label tRNA-Ile-tRNA-Phe

Feature Metadata

label_name: tRNA-Ile-tRNA-Phe
feature_type: igs
region: Genome
length_bp: 1941
variable_sites: 29
pi: 0.00996
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ile-tRNA-Phe_p1	GTTGGGCGCTTTAACCATTC	CCTCGTGTCACCAGTTCAAA	50.0	50.0	58.0	58.1	2007-2021 bp	1.0	80.7
tRNA-Ile-tRNA-Phe_p2	GTTGGGCGCTTTAACCATTC	TACCAGGATGATCCACAGGG	50.0	55.0	58.0	58.2	2070-2084 bp	1.0	79.5
tRNA-Ile-tRNA-Phe_p3	AGTTGGGCGCTTTAACCATT	CCTCGTGTCACCAGTTCAAA	45.0	50.0	58.4	58.1	2008-2022 bp	1.0	77.8
tRNA-Ile-tRNA-Phe_p4	TTGGGCGCTTTAACCATTCA	CCTCGTGTCACCAGTTCAAA	45.0	50.0	58.4	58.1	2006-2020 bp	1.0	77.7
tRNA-Ile-tRNA-Phe_p5	GTTGGGCGCTTTAACCATTC	AATCCTCGTGTCACCAGTTC	50.0	50.0	58.0	57.5	2010-2024 bp	1.0	77.4