Hotspot

tRNA-Asp-psbM

Genome · 31951-32682 · 732 bp

Back to Pseudotsuga

Pi 0.00911

MarkerSeek score 61.60

Primer available yes

Label tRNA-Asp-psbM

Feature Metadata

label_name: tRNA-Asp-psbM
feature_type: igs
region: Genome
length_bp: 732
variable_sites: 10
pi: 0.00911
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asp-psbM_p1	TTAGAGTACCGCCCTGTCAA	AGCTCGCTCGAGATTTGATC	50.0	50.0	58.1	57.9	835-840 bp	1.0	79.3
tRNA-Asp-psbM_p2	TTAGAGTACCGCCCTGTCAA	GCTCGCTCGAGATTTGATCT	50.0	50.0	58.1	57.9	834-839 bp	1.0	79.3
tRNA-Asp-psbM_p3	ATTGGTTAGAGTACCGCCCT	AGCTCGCTCGAGATTTGATC	50.0	50.0	58.1	57.9	840-845 bp	1.0	78.9
tRNA-Asp-psbM_p4	ATTGGTTAGAGTACCGCCCT	GCTCGCTCGAGATTTGATCT	50.0	50.0	58.1	57.9	839-844 bp	1.0	78.9
tRNA-Asp-psbM_p5	TAGAGTACCGCCCTGTCAAG	GCTCGCTCGAGATTTGATCT	55.0	50.0	58.5	57.9	833-838 bp	1.0	75.7