Hotspot

tRNA-Tyr-tRNA-Ser

Genome · 31235-31531 · 297 bp

Back to Pseudotsuga

Pi 0.047

MarkerSeek score 68.80

Primer available yes

Label tRNA-Tyr-tRNA-Ser

Feature Metadata

label_name: tRNA-Tyr-tRNA-Ser
feature_type: igs
region: Genome
length_bp: 297
variable_sites: 17
pi: 0.047
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Tyr-tRNA-Ser_p1	CTGGTTCAAATCCAGCTCGA	ATTTGAACCAGCGTAGGCAT	50.0	45.0	57.9	57.9	323-577 bp	1.0	86.4
tRNA-Tyr-tRNA-Ser_p2	CTGGTTCAAATCCAGCTCGA	TGGATTTGAACCAGCGTAGG	50.0	50.0	57.9	57.9	396-669 bp	1.0	86.1
tRNA-Tyr-tRNA-Ser_p3	AGTGGACCAGGACATCTCTC	TTGACAGGGCGGTACTCTAA	55.0	50.0	58.1	58.1	892-1216 bp	1.0	84.5
tRNA-Tyr-tRNA-Ser_p4	GTGGACCAGGACATCTCTCT	TTGACAGGGCGGTACTCTAA	55.0	50.0	58.1	58.1	891-1215 bp	1.0	84.5
tRNA-Tyr-tRNA-Ser_p5	CACGTGCGTGTGAAAAGAAA	TTGACAGGGCGGTACTCTAA	45.0	50.0	57.8	58.1	1121-1440 bp	1.0	84.3