Hotspot

tRNA-Thr-tRNA-Ser

Genome · 29209-30388 · 1180 bp

Back to Pseudotsuga

Pi 0.0119

MarkerSeek score 61.70

Primer available yes

Label tRNA-Thr-tRNA-Ser

Feature Metadata

label_name: tRNA-Thr-tRNA-Ser
feature_type: igs
region: Genome
length_bp: 1180
variable_sites: 21
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr-tRNA-Ser_p1	GAACCGATGACTTACGCCTT	TAGTGACCGAACGGAGAGAG	50.0	55.0	58.0	58.3	1334-1347 bp	1.0	79.2
tRNA-Thr-tRNA-Ser_p2	GAACCGATGACTTACGCCTT	CGGAGAGAGAGGGATTCGAA	50.0	55.0	58.0	58.3	1323-1336 bp	1.0	78.8
tRNA-Thr-tRNA-Ser_p3	CCATGGCGTTACTCTACCAC	TAGTGACCGAACGGAGAGAG	55.0	55.0	58.1	58.3	1313-1326 bp	1.0	78.8
tRNA-Thr-tRNA-Ser_p4	TGGCGTTACTCTACCACTGA	TAGTGACCGAACGGAGAGAG	50.0	55.0	58.1	58.3	1310-1323 bp	1.0	78.6
tRNA-Thr-tRNA-Ser_p5	CGTGTAAGACGGACATCCAG	TAGTGACCGAACGGAGAGAG	55.0	55.0	58.1	58.3	1399-1412 bp	1.0	78.5