Hotspot

ycf4-psaI

Genome · 19843-20385 · 543 bp

Back to Pseudotsuga

Pi 0.0148

MarkerSeek score 53.40

Primer available yes

Label ycf4-psaI

Feature Metadata

label_name: ycf4-psaI
feature_type: igs
region: Genome
length_bp: 543
variable_sites: 12
pi: 0.0148
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-psaI_p1	GAGCCATTTGGATCGACGAT	TTTCCGCTCTCCGAAATCAG	50.0	50.0	58.1	58.0	1022-1025 bp	1.0	80.5
ycf4-psaI_p2	AGGGGTATCAGGTTCTCACC	TTTCCGCTCTCCGAAATCAG	55.0	50.0	58.1	58.0	1150-1153 bp	1.0	80.4
ycf4-psaI_p3	ACTCGAAATACCGACCAGGA	TTTCCGCTCTCCGAAATCAG	50.0	50.0	58.2	58.0	1124-1127 bp	1.0	80.2
ycf4-psaI_p4	CGACGAGGGGAAATACCTTC	TTTCCGCTCTCCGAAATCAG	55.0	50.0	57.8	58.0	1417-1420 bp	1.0	79.5
ycf4-psaI_p5	TTCAGCAGCTTTCTGTTCGA	TTTCCGCTCTCCGAAATCAG	45.0	50.0	57.8	58.0	1514-1517 bp	1.0	79.3