Hotspot

psbJ-petA

Genome · 15438-16485 · 1048 bp

Back to Pseudotsuga

Pi 0.00703

MarkerSeek score 63.70

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: Genome
length_bp: 1048
variable_sites: 11
pi: 0.00703
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	TGGAAGGATCCCTCTTTGGT	AGCATCTGTCATTCTGGCAC	50.0	50.0	57.9	58.3	1242-1288 bp	1.0	78.5
psbJ-petA_p2	TACCACTGGAAGGATCCCTC	AGCATCTGTCATTCTGGCAC	55.0	50.0	57.8	58.3	1248-1294 bp	1.0	77.6
psbJ-petA_p3	TGGAAGGATCCCTCTTTGGT	GCATCTGTCATTCTGGCACA	50.0	50.0	57.9	58.5	1241-1287 bp	1.0	76.2
psbJ-petA_p4	CCACTGGAAGGATCCCTCTT	AGCATCTGTCATTCTGGCAC	55.0	50.0	58.4	58.3	1246-1292 bp	1.0	75.7
psbJ-petA_p5	TACCACTGGAAGGATCCCTC	GCATCTGTCATTCTGGCACA	55.0	50.0	57.8	58.5	1247-1293 bp	1.0	75.2