Hotspot

petL-psbE

Genome · 13452-14660 · 1209 bp

Back to Pseudotsuga

Pi 0.00401

MarkerSeek score 61.60

Primer available yes

Label petL-psbE

Feature Metadata

label_name: petL-psbE
feature_type: igs
region: Genome
length_bp: 1209
variable_sites: 6
pi: 0.00401
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-psbE_p1	GCGTGGAAGGACTGAATGAA	AATGTCAGCAAAGGAGCGTT	50.0	45.0	58.2	58.4	1091-1303 bp	1.0	79.8
petL-psbE_p2	GCGTGGAAGGACTGAATGAA	GTATTCCCAGCCATGCTCAG	50.0	55.0	58.2	58.4	1066-1278 bp	1.0	79.7
petL-psbE_p3	GCGTGGAAGGACTGAATGAA	TAATGTCAGCAAAGGAGCGT	50.0	45.0	58.2	57.5	1092-1304 bp	1.0	79.1
petL-psbE_p4	TAGGCGTGGAAGGACTGAAT	AATGTCAGCAAAGGAGCGTT	50.0	45.0	58.4	58.4	1094-1306 bp	1.0	77.8
petL-psbE_p5	TAGGCGTGGAAGGACTGAAT	GTATTCCCAGCCATGCTCAG	50.0	55.0	58.4	58.4	1069-1281 bp	1.0	77.8