Hotspot

ycf12-clpP

Genome · 8634-9052 · 419 bp

Back to Pseudotsuga

Pi 0.00636

MarkerSeek score 45.10

Primer available yes

Label ycf12-clpP

Feature Metadata

label_name: ycf12-clpP
feature_type: igs
region: Genome
length_bp: 419
variable_sites: 4
pi: 0.00636
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf12-clpP_p1	AGTCGATGGGATCATAGGGG	AGCATCGTTACGTGTTTCGA	55.0	45.0	58.1	57.9	1004-1092 bp	1.0	81.5
ycf12-clpP_p2	AGTCGATGGGATCATAGGGG	CTCGTTGAATGACCTCTCCG	55.0	55.0	58.1	58.1	1069-1157 bp	1.0	81.2
ycf12-clpP_p3	AGTCGATGGGATCATAGGGG	CGTTATGAAAATCTGCGGCC	55.0	50.0	58.1	57.9	982-1070 bp	1.0	81.1
ycf12-clpP_p4	AGTCGATGGGATCATAGGGG	GTTATGAAAATCTGCGGCCG	55.0	50.0	58.1	57.9	981-1069 bp	1.0	81.1
ycf12-clpP_p5	GGTTGTATCGGGCCCATTAG	AGCATCGTTACGTGTTTCGA	55.0	45.0	58.1	57.9	926 bp	0.333333	52.0